---
name: Fog City Bio
slug: fogcitybio
site: cro
website_domain: fogcitybio.com
summary: Specialized CRO for immunoassay development and optimization using Simoa, HTRF, AlphaLISA, Luminex, MSD ECL, and label-free kinetics platforms. Direct scientist engagement with rapid turnaround and handoff-ready protocols. Headquartered in San Francisco, United States.
tagline: Rapid immunoassay optimization with direct scientist engagement and handoff-ready protocols.
founded_year: 
employee_count_band: 
employee_count_label: 
parent_name: 
parent_slug: 
hq_country: US
tier2_domain_verified: false
updated_at: 2026-10-05T07:05:13.573Z
services_count: 9
modalities_count: 2
indications_count: 0
locations_count: 1
review_signal_gate: closed
generated_by: BioBM Editorial AI
---

# Fog City Bio

Rapid immunoassay optimization with direct scientist engagement and handoff-ready protocols.

Listed on cro.directory.

## Summary

Specialized CRO for immunoassay development and optimization using Simoa, HTRF, AlphaLISA, Luminex, MSD ECL, and label-free kinetics platforms. Direct scientist engagement with rapid turnaround and handoff-ready protocols. Headquartered in San Francisco, United States.

## At a glance

- HQ country: US

## Services

- CMC Analytical Development
- Analytical Method Development & Validation
- Bioanalysis
- Ligand-Binding Bioanalysis (Large Molecule)
- Immunogenicity Assays (ADA / NAb)
- Biomarker Assays
- Flow Cytometry Services
- In Vitro Pharmacology
- Preclinical In Vitro Studies

## Modalities

- Biologics (Monoclonal Antibodies)
- Biologics (Other)

## Locations

- Headquarters, San Francisco, California, United States

## Long description

## Overview

Fog City Bio is a specialized contract research organization (CRO) focused on immunoassay development, optimization, and characterization. Based at MBC BioLabs in the San Francisco Bay Area, the company works directly with biotech R&D teams to develop and validate protein quantitation assays, binding kinetics measurements, and cell-based functional assays.

## Core Services

### Immunoassay Development
- **ELISA**: Custom sandwich, competitive, and inhibition assays with optimization for specific sample matrices (serum, plasma, lysate, cell culture)
- **Ultra-Sensitive Detection**: Simoa (0.01–1 pg/mL), AlphaLISA (0.1–10 pg/mL), HTRF/TR-FRET (1–100 pg/mL) for rare biomarkers and low-abundance proteins
- **Multiplex Platforms**: Luminex xMAP (50-plex), MSD QuickPlex ECL (10-plex) for simultaneous quantitation of multiple analytes from limited sample volumes
- **Assay Troubleshooting & Rescue**: Rescue of commercial ELISA kits and development of fully custom assays

### Binding & Kinetics Assays
- Antibody affinity (KD) and kinetics (kon/koff) determination
- Label-free methods: Spectral Shift (SpS), Malvern WAVEsystem (GCI), NanoTemper MST
- Epitope binning

### Cell-Based Functional Assays
- Viability, proliferation, and cytotoxicity assays
- Immune cell function (activation, ADCC)
- ELISpot and cytokine secretion
- Seahorse XF Pro (OCR/ECAR) metabolic profiling
- xCELLigence RTCA (impedance-based live-cell analysis)
- High-content screening (Araceli Endeavor Ultra)

### Assay Characterization & Validation
- Rigorous precision, linearity, and spike-recovery testing
- Matrix validation in client-specific sample types
- Parallelism and accuracy assessment

### Technology Transfer & Documentation
- Handoff-ready protocols with full development reports and raw data
- Automation-ready workflows optimized for Opentrons Flex, Tecan Fluent, Gilson PLATEMASTER
- Machine-readable and human-readable protocol formats
- Scientific debrief calls and on-site training support

## Operational Approach

**Direct Scientist Engagement**: Projects are led by a dedicated scientist rather than a project manager, eliminating handoffs and coordination delays.

**Rapid Workflow**: Five-step process from initial fit call through final report and handoff (10-min fit call → CDA & materials → rapid DoE screen → optimize & finalize → report & handoff).

**Automation-Driven Development**: Uses Opentrons Flex and other liquid handlers to define robust, digital protocols that minimize operator variability and ensure reproducible tech transfer.

**Equipment & Infrastructure**: State-of-the-art, manufacturer-maintained equipment including Simoa SR-X, Luminex INTELLIFLEX, MSD QuickPlex, Tecan and BioTek plate readers, Cytation imaging, Seahorse XF Pro, xCELLigence RTCA, and advanced imaging systems (Araceli Endeavor Ultra, EVOS M7000, Refeyn TwoMP, confocal microscopy).

## Regulatory & Compliance

All work is conducted under strict IP confidentiality (CDA). Services are offered for research use only (RUO).

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_Profile last updated: 2026-10-05T07:05:13.573Z. Profile facts may include AI-assisted enrichment._

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